Dynamic reorganization of microtubules and microfilaments in flax cells during the resistance response to flax rust infection
Identifieur interne : 001816 ( Main/Exploration ); précédent : 001815; suivant : 001817Dynamic reorganization of microtubules and microfilaments in flax cells during the resistance response to flax rust infection
Auteurs : Issei Kobayashi [Australie] ; Yuhko Kobayashi [Australie] ; Adrienne R. Hardham [Australie]Source :
- Planta [ 0032-0935 ] ; 1994.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Teeft :
- Actin, Actin filaments, Actin microfilaments, Appressoria, Barley coleoptile cells, Bison, Cell biol, Compatible interaction, Compatible interactions, Confocal, Cultivar, Cytoskeletal, Cytoskeletal elements, Cytoskeletal reorganization, Cytoskeleton, Defence, Flax, Flax cells, Flax rust, Flax rust fungus, Fungal, Fungal infection, Fungal infection structures, Fungal structures, Fungus, Haustorial, Haustorial mother cell, Haustorial mother cells, Haustorium, Hosh, Host defence, Hypersensitive, Hypersensitive cell death, Hypersensitive reaction, Hypersensitive response, Hypha, Incompatible, Incompatible interaction, Infection, Infection hypha, Infection hyphae, Infection structures, Inoculated, Inoculation, Kobayashi, Leaf system, Lini, Lini strain, Littlefield, Long axis, Melampsora, Melampsora lini, Mesophyll, Mesophyll cell, Mesophyll cells, Microfilaments, Microtubule, Microtubule arrays, Microtubule reorganization, Necrotic, Necrotic cell, Neighbouring cells, Physiol, Plant cells, Plant cytoskeleton, Plant pathol, Plant physiol, Present study, Rearrangement, Resistance response, Stomatal pore, Substomatal, Substomatal vesicle, Substomatal vesicles, Time course, Time point, Uninfected, Uninoculated, Vesicle.
Abstract
Abstract: The cytoskeleton in plant cells is a dynamic structure that can rapidly respond to extracellular stimuli. Alteration of the organization of microtubules and actin microfilaments was examined in mesophyll cells of flax, Linum usitatissimum L., during attempted infection by the flax rust fungus, Melampsora lini (Ehrenb.) Lev. Flax leaves that had been inoculated with either a compatible (yielding a susceptible reaction) or an incompatible (yielding a resistant reaction) strain of M. lini were embedded in butyl-methylmethacrylate resin; sections of this material were immunofluorescently labelled with anti-tubulin or anti-actin and examined using confocal laser scanning microscopy. In uninfected leaves, microtubules in the mesophyll cells formed a transverse array in the cell cortex. Microfilaments radiated through the cytoplasm from the nucleus. In an incompatible interaction, microtubules and microfilaments were extensively reorganized in mesophyll cells that were in contact with fungal infection hyphae or haustorial mother cells before penetration of the cell by the infection peg. After the initiation of haustorium development, microtubules disappeared from the infected cells, and growth of the haustoria ceased. In an incompatible interaction, hypersensitive cell death occurred in more than 70% of infected cells but occurred in less than 20% of cells in compatible interactions. After the infected cell had undergone hypersensitive cell death, the cytoskeleton in neighbouring cells became focused on the walls shared with the necrotic cell. In compatible interactions, reorganization of the cytoskeleton was either not observed at all or was observed much less frequently up to 48 h after inoculation.
Url:
DOI: 10.1007/BF00199684
Affiliations:
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Le document en format XML
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Hardham</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, 2601, Canberra, ACT</wicri:regionArea><wicri:noRegion>ACT</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Australie</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Planta</title><title level="j" type="abbrev">Planta</title><idno type="ISSN">0032-0935</idno><idno type="eISSN">1432-2048</idno><imprint><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-SWLMH5L1-1">Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1994">1994</date><biblScope unit="vol" from="195" to="195">195</biblScope><biblScope unit="issue" from="2" to="2">2</biblScope><biblScope unit="page" from="237">237</biblScope><biblScope unit="page" to="247">247</biblScope></imprint><idno type="ISSN">0032-0935</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins</term><term>Cytoskeleton</term><term>Defense mechanism</term><term>Host agent relation</term><term>Hypersensitivity</term><term>Linum usitatissimum</term><term>Melampsora lini</term><term>Microfilament</term><term>Microtubule</term><term>Plant leaf</term><term>Resistance</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Actine</term><term>Cytosquelette</term><term>Feuille végétal</term><term>Hypersensibilité</term><term>Linum usitatissimum</term><term>Melampsora lini</term><term>Microfilament</term><term>Microtubule</term><term>Mécanisme défense</term><term>Relation hôte agent</term><term>Résistance</term><term>cv Bison</term><term>cv Hosh</term><term>cv RD9</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actin</term><term>Actin filaments</term><term>Actin microfilaments</term><term>Appressoria</term><term>Barley coleoptile cells</term><term>Bison</term><term>Cell biol</term><term>Compatible interaction</term><term>Compatible interactions</term><term>Confocal</term><term>Cultivar</term><term>Cytoskeletal</term><term>Cytoskeletal elements</term><term>Cytoskeletal reorganization</term><term>Cytoskeleton</term><term>Defence</term><term>Flax</term><term>Flax cells</term><term>Flax rust</term><term>Flax rust fungus</term><term>Fungal</term><term>Fungal infection</term><term>Fungal infection structures</term><term>Fungal structures</term><term>Fungus</term><term>Haustorial</term><term>Haustorial mother cell</term><term>Haustorial mother cells</term><term>Haustorium</term><term>Hosh</term><term>Host defence</term><term>Hypersensitive</term><term>Hypersensitive cell death</term><term>Hypersensitive reaction</term><term>Hypersensitive response</term><term>Hypha</term><term>Incompatible</term><term>Incompatible interaction</term><term>Infection</term><term>Infection hypha</term><term>Infection hyphae</term><term>Infection structures</term><term>Inoculated</term><term>Inoculation</term><term>Kobayashi</term><term>Leaf system</term><term>Lini</term><term>Lini strain</term><term>Littlefield</term><term>Long axis</term><term>Melampsora</term><term>Melampsora lini</term><term>Mesophyll</term><term>Mesophyll cell</term><term>Mesophyll cells</term><term>Microfilaments</term><term>Microtubule</term><term>Microtubule arrays</term><term>Microtubule reorganization</term><term>Necrotic</term><term>Necrotic cell</term><term>Neighbouring cells</term><term>Physiol</term><term>Plant cells</term><term>Plant cytoskeleton</term><term>Plant pathol</term><term>Plant physiol</term><term>Present study</term><term>Rearrangement</term><term>Resistance response</term><term>Stomatal pore</term><term>Substomatal</term><term>Substomatal vesicle</term><term>Substomatal vesicles</term><term>Time course</term><term>Time point</term><term>Uninfected</term><term>Uninoculated</term><term>Vesicle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The cytoskeleton in plant cells is a dynamic structure that can rapidly respond to extracellular stimuli. Alteration of the organization of microtubules and actin microfilaments was examined in mesophyll cells of flax, Linum usitatissimum L., during attempted infection by the flax rust fungus, Melampsora lini (Ehrenb.) Lev. Flax leaves that had been inoculated with either a compatible (yielding a susceptible reaction) or an incompatible (yielding a resistant reaction) strain of M. lini were embedded in butyl-methylmethacrylate resin; sections of this material were immunofluorescently labelled with anti-tubulin or anti-actin and examined using confocal laser scanning microscopy. In uninfected leaves, microtubules in the mesophyll cells formed a transverse array in the cell cortex. Microfilaments radiated through the cytoplasm from the nucleus. In an incompatible interaction, microtubules and microfilaments were extensively reorganized in mesophyll cells that were in contact with fungal infection hyphae or haustorial mother cells before penetration of the cell by the infection peg. After the initiation of haustorium development, microtubules disappeared from the infected cells, and growth of the haustoria ceased. In an incompatible interaction, hypersensitive cell death occurred in more than 70% of infected cells but occurred in less than 20% of cells in compatible interactions. After the infected cell had undergone hypersensitive cell death, the cytoskeleton in neighbouring cells became focused on the walls shared with the necrotic cell. In compatible interactions, reorganization of the cytoskeleton was either not observed at all or was observed much less frequently up to 48 h after inoculation.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Kobayashi, Issei" sort="Kobayashi, Issei" uniqKey="Kobayashi I" first="Issei" last="Kobayashi">Issei Kobayashi</name></noRegion><name sortKey="Hardham, Adrienne R" sort="Hardham, Adrienne R" uniqKey="Hardham A" first="Adrienne R." last="Hardham">Adrienne R. Hardham</name><name sortKey="Hardham, Adrienne R" sort="Hardham, Adrienne R" uniqKey="Hardham A" first="Adrienne R." last="Hardham">Adrienne R. Hardham</name><name sortKey="Kobayashi, Yuhko" sort="Kobayashi, Yuhko" uniqKey="Kobayashi Y" first="Yuhko" last="Kobayashi">Yuhko Kobayashi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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